SIRT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

67

Cat no : 19655-1-AP

Synonyms

SIR2, Regulatory protein SIR2 homolog 2, NAD-dependent protein defatty-acylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, EC:2.3.1.286

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Validation Data Gallery

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  WB analysis using 19655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19655-1-AP (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19655-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19655-1-AP (SIRT2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19655-1-AP

  IP result of anti-SIRT2 (IP:19655-1-AP, 4ug; Detection:19655-1-AP 1:800) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of rat brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IHC staining of rat brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IHC staining of human heart using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IF Staining of mouse cortex using 19655-1-AP

  IF results of SIRT2 (19655-1-AP) antibody with cortex slides of SITR2-WT and SIRT2-KO samples.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat brain tissue, human skeletal muscle tissue, mouse brain tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 61 publications below
• IHC: See 6 publications below
• IF: See 14 publications below
• IP: See 3 publications below
• CoIP: See 6 publications below

Product Information

19655-1-AP targets SIRT2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, zebrafish, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT2 fusion protein Ag7756
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC003547
• Gene Symbol: SIRT2
• Gene ID (NCBI): 22933
• RRID: AB_2878592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (10545947). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (12620231,16648462). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (12620231). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (12620231,10966460). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (16648462,12697818). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (12697818). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. This antibody recognizes the 37-45 KD SIRT2 proteins. This antibody is a specific antibody that it can't detect signal with SIRT2-KO samples.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
human,mouse	WB,IF	Neuron; Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.; Authors - Kelly A Chamberlain ; KO Validated
human	WB	J Clin Invest; SIRT2 protects peripheral neurons from cisplatin-induced injury by enhancing nucleotide excision repair.; Authors - Manchao Zhang
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
mouse	WB	Aging Cell; SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.; Authors - Yingying Zhao ; KD Validated

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