Anticorps Monoclonal anti-SIRT1

• Phare
• Validé par KD/KO

SIRT1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

48

CloneNo.

7F6G6

N° de cat : 60303-1-Ig

Synonymes

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

---

Galerie de données de validation

• 

  WB analysis using 60303-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Hek-293 cells using 60303-1-Ig

  WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells.

• 

  WB analysis using 60303-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60303-1-Ig

  IP Result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human breast cancer using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60303-1-Ig (SIRT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60303-1-Ig (SIRT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 60303-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig(SIRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules LNCaP, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60303-1-Ig cible SIRT1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRT1 Protéine recombinante Ag17677
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Masse moléculaire calculée: 747 aa, 82 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC012499
• Symbole du gène: SIRT1
• Identification du gène (NCBI): 23411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Publications

Species	Application	Title
	WB,IF	J Pineal Res; Melatonin Protects Mouse Testes from Palmitic Acid-Induced Lipotoxicity by Attenuating Oxidative Stress and DNA Damage in a SIRT1-Dependent Manner.; Authors - Dejun Xu
	WB,IF	Free Radic Biol Med; Menaquinone-4 prevents medication-related osteonecrosis of the jaw through the SIRT1 signaling-mediated inhibition of cellular metabolic stresses-induced osteoblast apoptosis; Authors - Yajun Cui
	IHC	Int J Mol Sci; Exposure to Bisphenol A Caused Hepatoxicity and Intestinal Flora Disorder in Rats.; Authors - Ruijing Liu
	WB	Int J Mol Sci; COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR.; Authors - Chen-Yu Zhang
pig	WB	J Cell Physiol; Y-box binding protein 1 influences zygotic genome activation by regulating N6-methyladenosine in porcine embryos; Authors - Wen-Jie Jiang
pig	IF	J Cell Physiol; Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.; Authors - Wenjun Zhou

Produits associés

P53 Polyclonal antibody

10442-1-AP

NF-κB p65 Polyclonal antibody

10745-1-AP

NCOR1 Polyclonal antibody

20018-1-AP

FOXO1 Polyclonal antibody

18592-1-AP

FOXO3A Polyclonal antibody

10849-1-AP

NFKB1,p105,p50 Polyclonal antibody

14220-1-AP