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- KD/KO Validated
FOXO3A Polyclonal antibody
FOXO3A Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 10849-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, Neuro-2a cells, PC-3 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 7 publications below|
|WB||See 64 publications below|
|IHC||See 8 publications below|
|IF||See 11 publications below|
|IP||See 1 publications below|
|CoIP||See 1 publications below|
10849-1-AP targets FOXO3A in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FOXO3A fusion protein Ag1289|
|Full Name||forkhead box O3|
|Calculated molecular weight||673 aa, 71 kDa|
|Observed molecular weight||72-97 kDa|
|GenBank accession number||BC021224|
|Gene ID (NCBI)||2309|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)
|Product Specific Protocols|
|WB protocol for FOXO3A antibody 10849-1-AP||Download protocol|
|IHC protocol for FOXO3A antibody 10849-1-AP||Download protocol|
|IF protocol for FOXO3A antibody 10849-1-AP||Download protocol|
|Click here to view our Standard Protocols|
PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis.
Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.
A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.
J Cell Physiol
Adiponectin regulates osteocytic MLO-Y4 cell apoptosis in a high-glucose environment through the AMPK/FoxO3a signaling pathway.
Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.
Am J Respir Cell Mol Biol
RANKL Mediates Muscle Atrophy and Dysfunction in a Cigarette Smoke-induced Model of Chronic Obstructive Pulmonary Disease.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Simona (Verified Customer) (03-03-2023)
The FOXO3A band is clearly visible in SH-SY5Y neuroblastoma cells, the last line, consisting of PC3 cells was considered as positive control but the signal was lower than SH-SY5Y. The product is useful.
Valeria (Verified Customer) (11-11-2020)
Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution.