FOXO3A Polyclonal antibody

• Featured Product
• KD/KO Validated

FOXO3A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

106

Reviews (3)

Cat no : 10849-1-AP

Synonyms

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A

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Validation Data Gallery

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  WB analysis of HEK-293 using 10849-1-AP

  WB result of FOXO3A antibody (10849-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO3A transfected HEK-293 cells.

• 

  WB analysis using 10849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10849-1-AP (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10849-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10849-1-AP (FOXO3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10849-1-AP

  IP result of anti-FOXO3A (IP:10849-1-AP, 4ug; Detection:10849-1-AP 1:8000) with HEK-293 cells lysate 920 ug.

• 

  IHC staining of human breast cancer using 10849-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10849-1-AP (FOXO3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10849-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10849-1-AP (FOXO3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10849-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10849-1-AP (FOXO3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10849-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10849-1-AP (FOXO3A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, Neuro-2a cells, PC-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 98 publications below
• IHC: See 15 publications below
• IF: See 16 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

10849-1-AP targets FOXO3A in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXO3A fusion protein Ag1289
• Full Name: forkhead box O3
• Calculated Molecular Weight: 673 aa, 71 kDa
• Observed Molecular Weight: 72-97 kDa
• GenBank Accession Number: BC021224
• Gene Symbol: FOXO3A
• Gene ID (NCBI): 2309
• RRID: AB_2247214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)

Publications

Species	Application	Title
human,mouse	WB,IP	J Hazard Mater; Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1; Authors - Hao Chen
human	WB	Nat Commun; PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis.; Authors - Shasha Yin
human	WB	Nat Commun; Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.; Authors - Chen Xu
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
mouse,human	WB,IF	Redox Biol; A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.; Authors - Qifeng Deng
	WB	Environ Pollut; Response of lipid metabolism, energy supply, and cell fate in yellowstripe goby (Mugilogobius chulae) exposed to environmentally relevant concentrations atorvastatin; Authors - Yufei Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Simona (Verified Customer) (03-03-2023)

The FOXO3A band is clearly visible in SH-SY5Y neuroblastoma cells, the last line, consisting of PC3 cells was considered as positive control but the signal was lower than SH-SY5Y. The product is useful.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y

FOXO3A Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y (Cat no:10849-1-AP)

FH

Valeria (Verified Customer) (11-11-2020)

Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human skeletal muscle sections (10um)

FOXO3A Antibody Immunofluorescence validation (1:50 dilution) in Human skeletal muscle sections (10um) (Cat no:10849-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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