NCOR1 Polyclonal antibody

NCOR1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 20018-1-AP

Synonyms

hCIT529I10, hN CoR, KIAA1047, N CoR, N CoR1, NCOR1, Nuclear receptor corepressor 1, TRAC1



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20018-1-AP targets NCOR1 in WB, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nuclear receptor co-repressor 1
Calculated molecular weight 270 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_006311
Gene symbol NCOR1
Gene ID (NCBI) 9611
RRIDAB_10665360
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCOR1, also named as KIAA1047 and TRAC1, belongs to the N-CoR nuclear receptor corepressors family. NCO1 mediates transcriptional repression by certain nuclear receptors. It is part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. The antibody recognizes the 103 kDa isoform of NCOR1.

Protocols

Product Specific Protocols
WB protocol for NCOR1 antibody 20018-1-APDownload protocol
IHC protocol for NCOR1 antibody 20018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Nat Commun

Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

Authors - Evangelia Lekka
chIP

PLoS Biol

Transcriptional programming of lipid and amino acid metabolism by the skeletal muscle circadian clock.

Authors - Kenneth Allen Dyar
mouseWB

iScience

WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation

Authors - Kai Song