- Featured Product
- KD/KO Validated
FOXO1 Polyclonal antibody
FOXO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 18592-1-AP
|Positive WB detected in
|DU 145 cells, HEK-293T cells, HepG2 cells, SGC-7901 cells, mouse brain tissue, rat brain tissue, mouse kidney tissue
|Positive IP detected in
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:1000-1:4000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18592-1-AP targets FOXO1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, sheep, pig
|Host / Isotype
|Rabbit / IgG
|FOXO1 fusion protein Ag13296
|forkhead box O1
|Calculated molecular weight
|655 aa, 70 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).
|Product Specific Protocols
|WB protocol for FOXO1 antibody 18592-1-AP
|IHC protocol for FOXO1 antibody 18592-1-AP
|IF protocol for FOXO1 antibody 18592-1-AP
|IP protocol for FOXO1 antibody 18592-1-AP
|FC protocol for FOXO1 antibody 18592-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hala (Verified Customer) (01-27-2023)
an specific band is observed around 20 KDa
Ruchi (Verified Customer) (07-08-2022)
I used the free sample antibody for immunofluorescence and I am happy with the results. I would like to order Proteintech products for my lab needs