FOXO1 Polyclonal antibody

FOXO1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, FC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 18592-1-AP

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A



Tested Applications

Positive WB detected inDU 145 cells, HEK-293T cells, HepG2 cells, SGC-7901 cells, mouse brain tissue, rat brain tissue, mouse kidney tissue
Positive IHC detected inhuman prostate cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18592-1-AP is FOXO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Hu sheep, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXO1 fusion protein Ag13296
Full Name forkhead box O1
Calculated molecular weight 655 aa, 70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC021981
Gene symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

Protocols

Product Specific Protocols
WB protocol for FOXO1 antibody 18592-1-APDownload protocol
IHC protocol for FOXO1 antibody 18592-1-APDownload protocol
FC protocol for FOXO1 antibody 18592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Molecules

Sweroside Alleviated LPS-Induced Inflammation via SIRT1 Mediating NF-κB and FOXO1 Signaling Pathways in RAW264.7 Cells.

Authors - Rui Wang
ratWB,IF

Cell Death Dis

Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.

Authors - Jitai Zhang
humanWB

Diabetes Metab Syndr Obes

Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4.

Authors - Hang Zhao
mouseChIP

J Biol Chem

Hepatic p53 is regulated by transcription factor FOXO1 and acutely controls glycogen homeostasis.

Authors - Moritz Oster
ratWB

Biomed Res Int

MiR-21-3p Plays a Crucial Role in Metabolism Alteration of Renal Tubular Epithelial Cells during Sepsis Associated Acute Kidney Injury via AKT/CDK2-FOXO1 Pathway.

Authors - Zhuoyong Lin
humanWB

Biochem Biophys Res Commun

AMPK alleviates endoplasmic reticulum stress by inducing the ER-chaperone ORP150 via FOXO1 to protect human bronchial cells from apoptosis.

Authors - Ji-Qiang Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ruchi (Verified Customer) (07-08-2022)

I used the free sample antibody for immunofluorescence and I am happy with the results. I would like to order Proteintech products for my lab needs

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: FOXO1
  • Cell Tissue Type: Mouse skin
FOXO1 Antibody Immunofluorescence validation (FOXO1 dilution) in Mouse skin (Cat no:18592-1-AP)