Anticorps Polyclonal de lapin anti-SEC61 Alpha

SEC61 Alpha Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 24935-1-AP

Synonymes

Sec61 alpha 2, SEC61A, SEC61A2, SEC61alpha

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Galerie de données de validation

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  WB analysis of mouse brain using 24935-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24935-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of U2OS using 24935-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61 Alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24935-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  IF Staining of HepG2 using 24935-1-AP

  Immunofluorescent analysis of HepG2 cells using 24935-1-AP (SEC61alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules U2OS, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24935-1-AP cible SEC61 Alpha dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC61 Alpha Protéine recombinante Ag20779
• Nom complet: Sec61 alpha 2 subunit (S. cerevisiae)
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC026179
• Symbole du gène: SEC61 Alpha
• Identification du gène (NCBI): 55176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

Publications

Species	Application	Title
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
rat	CoIP	iScience; The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors; Authors - Angela L. Whittsette

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a band around 60 kd, but it also recognized a similar band in solo loading buffer.

• Applications: Western Blot

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