Anticorps Polyclonal de lapin anti-RAD51

• Phare
• Validé par KD/KO

RAD51 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

68

Avis (2)

N° de cat : 14961-1-AP

Synonymes

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

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Galerie de données de validation

• 

  WB analysis using 14961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14961-1-AP

  WB result of RAD51 antibody (14961-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells.

• 

  WB analysis of HeLa using 14961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 56 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

14961-1-AP cible RAD51 dans les applications de WB, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD51 Protéine recombinante Ag6823
• Nom complet: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC001459
• Symbole du gène: RAD51
• Identification du gène (NCBI): 5888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Publications

Species	Application	Title
mouse		Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Nucleic Acids Res; LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.; Authors - Liping Shen ; KD Validated
human	WB,CoIP	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	WB	Int J Biol Macromol; Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation; Authors - Hong-Yu Tao
human	WB	Oncogene; BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.; Authors - Mengdong Ni

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Resha (Verified Customer) (10-11-2023)

The antibody detected band at 37KDa for Rad51 protein. We used 1:1000 dilution for 20ug of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

FH

ksh (Verified Customer) (01-17-2022)

This Antibody works for WB (1:1000) and IF (1:200).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

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