XRCC2 Polyclonal antibody

• 

  WB analysis of K-562 using 20285-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20285-1-AP (XRCC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20285-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20285-1-AP (XRCC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 20285-1-AP

  IP result of anti-XRCC2 (IP:20285-1-AP, 4ug; Detection:20285-1-AP 1:500) with K-562 cells lysate 3200ug.

XRCC2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 20285-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

DNA repair protein XRCC2, XRCC2

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Tested Applications

• Positive WB detected in: K-562 cells, A375 cells, HEK-293 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20285-1-AP targets XRCC2 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14011

  Product name: Recombinant human XRCC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-280 aa of BC042137

  Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC

• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 2
• Calculated Molecular Weight: 280 aa, 32 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC042137
• Gene Symbol: XRCC2
• Gene ID (NCBI): 7516
• RRID: AB_2878662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Struct Mol Biol; Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development; Authors - Nazareno Bona
human	WB	PLoS One; The dysregulation of PARP9 expression is linked to apoptosis and DNA damage in gastric cancer cells; Authors - Yating Li