Anticorps Polyclonal de lapin anti-RAB1A

• Phare
• Validé par KD/KO

RAB1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 11671-1-AP

Synonymes

YPT1 related protein, YPT1, Ras-related protein Rab-1A, Ras related protein Rab 1A, RAB1

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Galerie de données de validation

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  WB analysis using 11671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

• 

  WB analysis using 11671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11671-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11671-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 11671-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11671-1-AP

  IP result of anti-RAB1A (IP:11671-1-AP, 4ug; Detection:11671-1-AP 1:4000) with A549 cells lysate 920 ug.

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  IP experiment of C2C12 using 11671-1-AP

  IP result of anti-RAB1A (IP:11671-1-AP, 4ug; Detection:11671-1-AP 1:4000) with C2C12 cells lysate 1280 ug.

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  IHC staining of human lung cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11671-1-AP (RAB1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 11671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using RAB1A antibody (11671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of NIH/3T3 using 11671-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 11671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of C2C12 using 11671-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human RAB1A (11671-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules NIH/3T3, cellules U-251, cellules U2OS, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: cellules A549, cellules C2C12
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12, cellules A549, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Informations sur le produit

11671-1-AP cible RAB1A dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB1A Protéine recombinante Ag2264
• Nom complet: RAB1A, member RAS oncogene family
• Masse moléculaire calculée: 205 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC000905
• Symbole du gène: RAB1A
• Identification du gène (NCBI): 5861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

Publications

Species	Application	Title
human	WB, IP	Cancer Cell; Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.; Authors - Janice D Thomas ; KD Validated
human	IP	EMBO J; Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.; Authors - Chantal Sellier
mouse	WB	Hum Mol Genet; Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.; Authors - Gaynor A Smith
human	WB,IHC,IF	Cancer Lett; Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer.; Authors - Tinglei Huang ; KD Validated
mouse	WB	Mol Cell Proteomics; Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.; Authors - Campbell-Valois François-Xavier FX
mouse	IF	Hum Mol Genet; Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.; Authors - Mukesh Gautam

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (02-22-2024)

recognized a band around the expected MW, but this antibody gave a dirty background.

• Applications: Western Blot

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