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RAB6A Polyclonal antibody
RAB6A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10187-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
10187-2-AP targets RAB6A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB6A fusion protein Ag0240 |
| Full Name | RAB6A, member RAS oncogene family |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC003617 |
| Gene Symbol | RAB6A |
| Gene ID (NCBI) | 5870 |
| RRID | AB_2175463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB6A antibody 10187-2-AP | Download protocol |
| IF protocol for RAB6A antibody 10187-2-AP | Download protocol |
| IP protocol for RAB6A antibody 10187-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. | ||
J Neuroimmunol Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report. | ||
Neurosci Lett Absence of motor impairments or pathological changes in TMEM230 knockout rats | ||
Development A crucial role for Arf6 in the response of commissural axons to Slit.
| ||
Nat Commun SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (01-25-2019) | Very good, both for WB and IF assays.By WB, it gives a strong specific band at the expected molecular weight; by IF, PFA fixation, it labels Rab6 at the Golgi and the cilia.
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