RAB5A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB5A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

35

Reviews (4)

Cat no : 11947-1-AP

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

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Validation Data Gallery

• 

  WB analysis using 11947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11947-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11947-1-AP (RAB5A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11947-1-AP

  IP result of anti-RAB5A (IP:11947-1-AP, 4ug; Detection:11947-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.

• 

  IHC staining of human gliomas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11947-1-AP (RAB5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 11947-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11947-1-AP (RAB5A Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 25 publications below
• IHC: See 6 publications below
• IF: See 16 publications below
• IP: See 2 publications below

Product Information

11947-1-AP targets RAB5A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB5A fusion protein Ag2549
• Full Name: RAB5A, member RAS oncogene family
• Calculated Molecular Weight: 215 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001267
• Gene Symbol: RAB5A
• Gene ID (NCBI): 5868
• RRID: AB_2269388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
human	WB,IP	Autophagy; Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.; Authors - Amy H Ponsford
mouse	WB	Autophagy; RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.; Authors - Xin Jin
mouse	WB,IHC	Diabetes; Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.; Authors - Linyan Shen ; KD Validated
rat	WB, IF	Sci Signal; Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.; Authors - Kong-Yan Wu
human	WB,IF,IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sophy (Verified Customer) (02-27-2023)

The antibody was used at 1:1000 for western blot. The signal showed up but not as strong as expected. But since this was the only antibody purchased for Rab5A, I had no comparison to antibodies to other vendors.

• Applications: Western Blot
• Cell Tissue Type: NSCLC cells

FH

Xin (Verified Customer) (01-24-2022)

Very good antibody in WB (25 kD) with a high titer

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse brain lysate

FH

Tom (Verified Customer) (11-13-2020)

HEK293T cell extracts (10ug/lane). Primary antibody (1:2000) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-rabbit HRP secondary antibody (1:10,000) incubated for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RAB5A Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11947-1-AP)

FH

Aamir (Verified Customer) (01-19-2020)

Used in HEK cells. Good signal for WB and IF

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: HEKs

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