Anticorps Polyclonal de lapin anti-Periostin

• Phare
• Validé par KD/KO

Periostin Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

28

N° de cat : 19899-1-AP

Synonymes

OSF 2, OSF2, Osteoblast specific factor 2, PDLPOSTN, Periostin, PN, POSTN, RP11 412K4.1

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Galerie de données de validation

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  IHC staining of human colon using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19899-1-AP (Periostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19899-1-AP (Periostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer de la peau humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 8 publications below
• IF: See 15 publications below

Informations sur le produit

19899-1-AP cible Periostin dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Periostin Protéine recombinante Ag13791
• Nom complet: periostin, osteoblast specific factor
• Masse moléculaire calculée: 93 kDa
• Numéro d’acquisition GenBank: BC106710
• Symbole du gène: Periostin
• Identification du gène (NCBI): 10631
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot,PMID: 21997759).

Publications

Species	Application	Title
mouse	IF	Clin Exp Hypertens; Nuclear receptor subfamily 1 group D member 1 suppresses the proliferation, migration of adventitial fibroblasts, and vascular intimal hyperplasia via mammalian target of rapamycin complex 1/β-catenin pathway; Authors - Ke Peng
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
rat	IF	Antioxidants (Basel); MicroRNA-4732-3p Is Dysregulated in Breast Cancer Patients with Cardiotoxicity, and Its Therapeutic Delivery Protects the Heart from Doxorubicin-Induced Oxidative Stress in Rats; Authors - Rafael Sánchez-Sánchez
human,mouse	WB,IF	J Nanobiotechnology; Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.; Authors - Na Zhao
human	IF	Cell Death Dis; Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.; Authors - Binbin Yu
human	IF	Stem Cell Res Ther; Adipose-derived stromal/stem cells are verified to be potential seed candidates for bio-root regeneration in three-dimensional culture.; Authors - Yu Yuan

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