Anticorps Polyclonal de lapin anti-PGRMC1

• Phare
• Validé par KD/KO

PGRMC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 12990-1-AP

Synonymes

HPR6.6, MPR, PGRMC, PGRMC1

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Galerie de données de validation

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  WB analysis of HEK-293 using 12990-1-AP

  WB result of PGRMC1 antibody (12990-1-AP; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells.

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  WB analysis using 12990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12990-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 12990-1-AP

  IP result of anti-PGRMC1 (IP:12990-1-AP, 3ug; Detection:12990-1-AP 1:1000) with MCF-7 cells lysate 1600ug.

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 12990-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PGRMC1 antibody (12990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules MCF-7, tissu hépatique de souris, tissu ovarien de souris, tissu rénal de souris
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain, tissu ovarien de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

12990-1-AP cible PGRMC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGRMC1 Protéine recombinante Ag3643
• Nom complet: progesterone receptor membrane component 1
• Masse moléculaire calculée: 195 aa, 22 kDa
• Poids moléculaire observé: 22-28 kDa
• Numéro d’acquisition GenBank: BC034238
• Symbole du gène: PGRMC1
• Identification du gène (NCBI): 10857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

Publications

Species	Application	Title
human	WB	EBioMedicine; The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes.; Authors - Uyen B Chu ; KO Validated
human	IHC	Int J Cancer; Elevated progesterone receptor membrane component 1/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients.; Authors - Mir Shakeel U R SU
rat	WB	Transl Psychiatry; A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.; Authors - H L Cai
rat	WB	Front Pharmacol; A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.; Authors - Ting Cao
human	WB	Biol Reprod; Hyaluronic Acid Promotes the Expression of Progesterone Receptor Membrane Component 1 via Epigenetic Silencing of miR-139-5p in Granulosa Cells.; Authors - Guangfeng Zhao
rat	WB	J Cell Physiol; Progesterone maintains the status of granulosa cells and slows follicle development partly through PGRMC1.; Authors - Xiao-Hua Yuan

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