Anticorps Monoclonal anti-OCT4

OCT4 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

CloneNo.

1C4B6

N° de cat : 60242-1-Ig

Synonymes

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

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Galerie de données de validation

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  WB analysis using 60242-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60242-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60242-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60242-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 60242-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60242-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60242-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60242-1-Ig (OCT4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60242-1-Ig (OCT4 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules L02, cellules NCCIT, cellules Neuro-2a, cellules NIH/3T3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

60242-1-Ig cible OCT4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OCT4 Protéine recombinante Ag1794
• Nom complet: POU class 5 homeobox 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 50-52 kDa
• Numéro d’acquisition GenBank: BC020712
• Symbole du gène: POU5F1
• Identification du gène (NCBI): 5460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).

Publications

Species	Application	Title
human	WB,IHC	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
human	WB	J Hematol Oncol; A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.; Authors - Shuiqing Liu
human	WB	Theranostics; Sulforaphane Inhibits the Acquisition of Tobacco Smoke-Induced Lung Cancer Stem Cell-Like Properties via the IL-6/ΔNp63α/Notch Axis.; Authors - Chunfeng Xie
human	WB	Cancer Commun (Lond); KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression.; Authors - Haoran Wei
human	WB	Front Oncol; PIWIL1 Drives Chemoresistance in Multiple Myeloma by Modulating Mitophagy and the Myeloma Stem Cell Population.; Authors - Yajun Wang
human	IF	Stem Cell Res; Generation of a human induced pluripotent stem cell line (INNDSUi003-A) derived from patient with Becker muscular dystrophy (BMD).; Authors - Xinbo Ji

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