Lin28A-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

Lin28A-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (2)

Cat no : 16177-1-AP

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Lin28A-specific, Protein lin 28 homolog A, ZCCHC1

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Validation Data Gallery

• 

  WB analysis of hESC using 16177-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16177-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 16177-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16177-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16177-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: hESC cells, human brain tissue, human heart tissue, NCCIT cell, mouse liver tissue
• Positive IHC detected in: human small intestine tissue, human ovary tissue, human heart tissue, human testis tissue, human spleen tissue, human lung tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

16177-1-AP targets Lin28A-specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lin-28 homolog (C. elegans)
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_024674
• Gene Symbol: LIN28
• Gene ID (NCBI): 79727
• RRID: AB_2135038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.

Publications

Species	Application	Title
human	WB	Cell Death Differ; LIN28A enhances regenerative capacity of human somatic tissue stem cells via metabolic and mitochondrial reprogramming.; Authors - Kelvin Pieknell
human	WB	Cancer Res; Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.; Authors - Domenico Albino
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang ; KD Validated
human	WB	EMBO Rep; The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse.; Authors - Wenwen Wang
human	WB	Cell Death Dis; Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.; Authors - Qin He
human	WB	J Virol; Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.; Authors - Ali Naushad N

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carolina (Verified Customer) (09-26-2019)

Antibody was more specific than the previous Lin28A antibody.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: Human Testis

FH

KUNHUA (Verified Customer) (02-01-2019)

Got a very strong band at 25-37kd with 1:1000 concentration.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human erythroid progenitor