EWS Polyclonal antibody

EWS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 55191-1-AP

Synonyms

EWS, EWS oncogene, EWSR1, RNA binding protein EWS

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Validation Data Gallery

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  WB analysis using 55191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 55191-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55191-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse testis tissue, Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

55191-1-AP targets EWS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Ewing sarcoma breakpoint region 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: NM_005243
• Gene Symbol: EWS
• Gene ID (NCBI): 2130
• RRID: AB_10860907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Nucleic Acids Res; The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.; Authors - Huiwu Ouyang
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
mouse	IF	J Reprod Dev; Lipid droplets synthesized during luteinization are degraded after pregnancy; Authors - Junichiro Mitsui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manohar (Verified Customer) (12-18-2021)

Overnight primary antibody using milk to block the blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293