Tested Applications
| Positive WB detected in | K-562 cells, mouse testis tissue, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55191-1-AP targets EWS in WB, IHC, IF, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Ewing sarcoma breakpoint region 1 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 68-70 kDa |
| GenBank Accession Number | NM_005243 |
| Gene Symbol | EWS |
| Gene ID (NCBI) | 2130 |
| RRID | AB_10860907 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EWS antibody 55191-1-AP | Download protocol |
| WB protocol for EWS antibody 55191-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EWS Polyclonal antibody (Cat# 55191-1-AP) has 8 citations published in iMeta, Nucleic Acids Research, Advanced Science, Nature Structural & Molecular Biology, Cell Reports, Molecular Therapy Nucleic Acids, Acta Biochimica et Biophysica Sinica, and Journal of Reproduction and Development. Research fields span leukemia, RNA/miRNA processing, meiosis, ALS, lung cancer, hypoxia-cardiology, intestinal homeostasis, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Imeta Homoharringtonine suppresses acute myeloid leukemia progression by orchestrating EWSR1 phase separation in an m(6)A-YTHDF2-dependent mechanism.
| ||
Nucleic Acids Res The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells. | ||
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The user applied it for Western Blot on HEK293 cells at a 1:1000 dilution, using an overnight primary antibody incubation with milk for blocking. Overall, the feedback is positive, indicating the antibody performed well under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Manohar (Verified Customer) (12-18-2021) | Overnight primary antibody using milk to block the blot.
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