Anticorps Polyclonal de lapin anti-NNMT

• Phare
• Validé par KD/KO

NNMT Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (2)

N° de cat : 15123-1-AP

Synonymes

Nicotinamide N-methyltransferase, EC:2.1.1.1

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Galerie de données de validation

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  WB analysis of mouse liver using 15123-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15123-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15123-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15123-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15123-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15123-1-AP

  IP result of anti-NNMT (IP:15123-1-AP, 4ug; Detection:15123-1-AP 1:300) with HepG2 cells lysate 2400ug.

• 

  IF Staining of HepG2 using 15123-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15123-1-AP (NNMT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15123-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NNMT (15123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

15123-1-AP cible NNMT dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NNMT Protéine recombinante Ag7197
• Nom complet: nicotinamide N-methyltransferase
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC000234
• Symbole du gène: NNMT
• Identification du gène (NCBI): 4837
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Rep; Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis; Authors - Jia Yan
human	WB,IHC,IF	Oncogene; Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids; Authors - Hui Zhao
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
human	WB	Mol Oncol; Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.; Authors - Jie Li ; KD Validated
human	WB	Front Cell Infect Microbiol; Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.; Authors - Fengxue Geng
human	IHC	Sci Rep; The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease.; Authors - Rina Takahashi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

This antibody worked for immunofluorescence with a little background

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse brain

FH

Johnny (Verified Customer) (01-07-2022)

products from proteintech have always been reliable.

• Applications: Western Blot

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