MTHFD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTHFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 10794-1-AP

Synonyms

MTHFD, MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed

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Validation Data Gallery

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  WB analysis using 10794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 10794-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10794-1-AP

  IP result of anti-MTHFD1 (IP:10794-1-AP, 5ug; Detection:10794-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10794-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (10794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HuH-7 cells, human brain tissue, K-562 cells, mouse kidney tissue, MCF-7 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below

Product Information

10794-1-AP targets MTHFD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTHFD1 fusion protein Ag1225
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC009806
• Gene Symbol: MTHFD1
• Gene ID (NCBI): 4522
• RRID: AB_2147391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Publications

Species	Application	Title
human	WB	Nature; Oxidative stress inhibits distant metastasis by human melanoma cells.; Authors - Elena Piskounova ; KD Validated
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Redox Biol; Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.; Authors - Chang Shao
human	WB	Proc Natl Acad Sci U S A; Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.; Authors - Danielle E Anderson ; KD Validated
mouse	WB	Dev Cell; Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.; Authors - Yejun Zou
human	WB	Cell Death Dis; MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma; Authors - Jinqiu Guan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: liver, kidney, Hippocampus

MTHFD1 Antibody Western Blot, validation (1:500 dilution) in liver, kidney, Hippocampus (Cat no:10794-1-AP)