Thymidylate synthase Polyclonal antibody

• Featured Product
• KD/KO Validated

Thymidylate synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 15047-1-AP

Synonyms

TYMS, TMS, thymidylate synthetase, OK/SW-cl.29, HsT422

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Validation Data Gallery

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  WB analysis of HEK-293 using 15047-1-AP

  WB result of Thymidylate synthase antibody (15047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thymidylate synthase transfected HEK-293 cells.

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  WB analysis using 15047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 15047-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15047-1-AP

  IP result of anti-Thymidylate synthase (IP:15047-1-AP, 3ug; Detection:15047-1-AP 1:600) with HeLa cells lysate 3200ug.

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  IHC staining of human endometrial cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15047-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Thymidylate synthase antibody (15047-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HEK-293 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human endometrial cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 5 publications below

Product Information

15047-1-AP targets Thymidylate synthase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Thymidylate synthase fusion protein Ag7027
• Full Name: thymidylate synthetase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC002567
• Gene Symbol: TYMS
• Gene ID (NCBI): 7298
• RRID: AB_2210721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Publications

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
		Nat Commun; CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.; Authors - Helena Almqvist
human	WB	Cancer Res; 5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.; Authors - Yiqing Zhao
human	WB	Cell Rep; The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.; Authors - Gerta Hoxhaj
human	WB	Cell Chem Biol; CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.; Authors - Ying Yu Liang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang ; KD Validated

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