MTR Polyclonal antibody

MTR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 25896-1-AP

Synonyms

cblG, Methionine synthase, MS, MTR

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Validation Data Gallery

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  WB analysis of A375 using 25896-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 25896-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A375 using 25896-1-AP

  Immunofluorescent analysis of A375 cells using 25896-1-AP (MTR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, mouse pancreas tissue
• Positive IHC detected in: human pancreas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 25896-1-AP is MTR Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTR fusion protein Ag23156
• Full Name: 5-methyltetrahydrofolate-homocysteine methyltransferase
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC130616
• Gene Symbol: MTR
• Gene ID (NCBI): 4548
• RRID: AB_2880287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
mouse	WB	Clin Epigenetics; A transgenic mice model of retinopathy of cblG-type inherited disorder of one-carbon metabolism highlights epigenome-wide alterations related to cone photoreceptor cells development and retinal metabolism; Authors - Karim Matmat
mouse	WB	Nutrients; Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice.; Authors - Karen E Christensen
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto