Anticorps Polyclonal de lapin anti-MVP/LRP

• Phare
• Validé par KD/KO

MVP/LRP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 16478-1-AP

Synonymes

LRP, major vault protein, MVP, MVP/LRP, VAULT1

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Galerie de données de validation

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  WB analysis using 16478-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16478-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16478-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 16478-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 16478-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 16478-1-AP

  IP result of anti-MVP/LRP (IP:16478-1-AP, 4ug; Detection:16478-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human lung cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16478-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MVP/LRP antibody (16478-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules SH-SY5Y, RAW264.7, tissu cérébral humain, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain, tissu de cancer du poumon humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16478-1-AP cible MVP/LRP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MVP/LRP Protéine recombinante Ag9593
• Nom complet: major vault protein
• Masse moléculaire calculée: 893 aa, 99 kDa
• Poids moléculaire observé: 99-104 kDa
• Numéro d’acquisition GenBank: BC015623
• Symbole du gène: LRP
• Identification du gène (NCBI): 9961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

Publications

Species	Application	Title
mouse	WB	Cell; The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.; Authors - Deniz Simsek
human	WB,IHC,IF,IP	Drug Resist Updat; CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis; Authors - Jing Wu ; KD Validated
human	WB	Nat Commun; MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.; Authors - Yun Teng ; KO Validated
mouse	WB	Front Cell Dev Biol; Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.; Authors - Xinlei Li
human	WB	Front Cell Dev Biol; Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.; Authors - Xubin Dong ; KD Validated
mouse	WB	Cell Commun Signal; Differential RNA packaging into small extracellular vesicles by neurons and astrocytes.; Authors - Xuan Luo

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