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PTEN Polyclonal antibody

PTEN Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 22034-1-AP


10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, mouse testis tissue, NIH/3T3 cells, HEK-293T cells, C6 cells, rat testis tissue, MCF-7 cells, LNCaP cells, Jurkat cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22034-1-AP targets PTEN in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274
Full Name phosphatase and tensin homolog
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 55 kDa, 68 kDa
GenBank Accession NumberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.

What is the molecular weight of PTEN?

PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a 

C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize 

two isoforms with MWs of 55kDa and 68 kDa.

What is the function of PTEN?

Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression 

through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3) 

by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating 

activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis, 

PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be 

regulated by posttranslational modifications, including acetylation for decreased catalytic activity and 

ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity 

(PMID: 24656806).

What is the role of PTEN in cancer?

PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate 

groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer 

(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function 

in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a 

group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These 

disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of 

the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and 

an increased risk of developing tumors, both benign and malignant (PMID: 26564076).


Product Specific Protocols
WB protocol for PTEN antibody 22034-1-APDownload protocol
IHC protocol for PTEN antibody 22034-1-APDownload protocol
IP protocol for PTEN antibody 22034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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