YBX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YBX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

56

Reviews (4)

Cat no : 20339-1-AP

Synonyms

EFI A, DNA-binding protein B, DBPB, CCAAT-binding transcription factor I subunit A, CBF-A

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Validation Data Gallery

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  WB analysis using 20339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20339-1-AP (YBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20339-1-AP (YBX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20339-1-AP (YBX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 20339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20339-1-AP (YBX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20339-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using YBX1 antibody (20339-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells, NIH/3T3 cells
• Positive IHC detected in: human renal cell carcinoma tissue, human placenta tissue, human breast cancer tissue, human prostate cancer tissue, human tonsillitis tissue, mouse colon tissue, rat colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 42 publications below
• IHC: See 7 publications below
• IF: See 17 publications below
• IP: See 3 publications below
• CoIP: See 7 publications below
• ChIP: See 2 publications below
• RIP: See 9 publications below

Product Information

20339-1-AP targets YBX1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YBX1 fusion protein Ag14129
• Full Name: Y box binding protein 1
• Calculated Molecular Weight: 324 aa, 36 kDa
• Observed Molecular Weight: 36-56 kDa
• GenBank Accession Number: BC010430
• Gene Symbol: YBX1
• Gene ID (NCBI): 4904
• RRID: AB_10665424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Y box binding protein 1(YBX1) is one of cis-acting elements which has a role in regulation of the expression of HLA class II genes. YBX1 is great of importance in regulation of PTP1B expression. It can bind to splice sites mediate pre-mRNA alternative splicing regulation. Also, it involves the regulation of translation by modulation of the interaction between the mRNA and eukaryotic initiation factors. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. The calculated molecular weight of YBX1 is 36 kDa, but modified YBX1 is about 36-56 kDa. (PMID: 27384875 )

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
mouse	IF	Sci Adv; Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.; Authors - Chunxia Zhang
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei ; KD Validated
human	ChIP	Cell Death Differ; YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer.; Authors - Dewei Jiang ; KD Validated
human	WB,IF	Cell Rep; Coordinated post-transcriptional control of oncogene-induced senescence by UNR/CSDE1.; Authors - Rosario Avolio ; KD Validated
human	WB	Nucleic Acids Res; YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression.; Authors - Shawn M Lyons ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Detects protein recruited to sodium arsenite induced stress granules (see image). 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h at RT incubation.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

YBX1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:20339-1-AP)

FH

Sun (Verified Customer) (01-05-2023)

a good antibody. YBX1 is a quite abundant protein and this antibody detects it pretty well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

FH

Meliti (Verified Customer) (08-05-2020)

Not vey clean in terms of the background but gives a nice band corresponding to YBX1

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293

YBX1 Antibody Western Blot validation (1:3000 dilution) in HEK293 (Cat no:20339-1-AP)

FH

H (Verified Customer) (04-06-2020)

This antibody works well in my hands using HEK293T cell lysates. I used LICOR anti-rabbit IRDye800 secondary antibody coupled with Odyssey Clx detection.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

YBX1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:20339-1-AP)

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