Anticorps Polyclonal de lapin anti-MPP8

• Phare
• Validé par KD/KO

MPP8 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 16796-1-AP

Synonymes

HSMPP8, M phase phosphoprotein 8, MPHOSPH8, MPP8, TWA3

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Galerie de données de validation

• 

  WB analysis using 16796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16796-1-AP (MPP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16796-1-AP

  IP result of anti-MPP8 (IP:16796-1-AP, 4ug; Detection:16796-1-AP 1:20000) with HEK-293 cells lysate 1400 ug.

• 

  IF Staining of U2OS using 16796-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MPP8 antibody (16796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NIH/3T3, cellules U-251, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

16796-1-AP cible MPP8 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPP8 Protéine recombinante Ag10268
• Nom complet: M-phase phosphoprotein 8
• Masse moléculaire calculée: 860 aa, 97 kDa
• Poids moléculaire observé: 97-105 kDa
• Numéro d’acquisition GenBank: BC003542
• Symbole du gène: MPP8
• Identification du gène (NCBI): 54737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) [PMID: 18726070].

Publications

Species	Application	Title
human	WB	Nature; Genome surveillance by HUSH-mediated silencing of intronless mobile elements.; Authors - Marta Seczynska
human	WB,IF	Science; GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.; Authors - Iva A Tchasovnikarova ; KD Validated
human	WB	Nat Genet; Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.; Authors - Iva A Tchasovnikarova
	ChIP	Nat Genet; Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia.; Authors - Zhimin Gu
mouse	WB,IP	Mol Cell; Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression.; Authors - William Garland
human	WB	Nat Microbiol; Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.; Authors - Leonid Yurkovetskiy ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

Great antibody to IP MPP8, working well on WB as well (1/1000, 1h at RT) in HeLa cells and Jurkat cells.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa, Jurkat

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