Muskelin Polyclonal antibody

• Featured Product
• KD/KO Validated

Muskelin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (2)

Cat no : 14735-1-AP

Synonyms

MKLN1, Muskelin, TWA2

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Validation Data Gallery

• 

  WB analysis of Y79 using 14735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14735-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14735-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14735-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 14735-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Y79 cells, Jurkat cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

14735-1-AP targets Muskelin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Muskelin fusion protein Ag6147
• Full Name: muskelin 1, intracellular mediator containing kelch motifs
• Calculated Molecular Weight: 735 aa, 85 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC067825
• Gene Symbol: Muskelin
• Gene ID (NCBI): 4289
• RRID: AB_2142504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP,IF	Neuron; Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.; Authors - Frank F Heisler ; KO Validated
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman
human	WB,IP	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (12-31-2021)

This antibody is ok, but not the best. At least, KO validation. The weak signal could be due to the low protein expression level though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and several others

Muskelin Antibody Western Blot validation (1:1000 dilution) in HEK293T and several others (Cat no:14735-1-AP)

FH

H (Verified Customer) (04-05-2020)

Primary antibody: 1:1000 dilution in 5% BSA, over night at 4CSecondary antibody: Rabbit IRDye800Detection: odyssey ClxI was able to detect the band in HEK293T and HCT116 cells. However, the band intensity was not that strong.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Muskelin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14735-1-AP)