TSFM Polyclonal antibody

• Featured Product
• KD/KO Validated

TSFM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11701-1-AP

Synonyms

EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3

---

Validation Data Gallery

• 

  WB analysis of HeLa using 11701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11701-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11701-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 11701-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 11701-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

11701-1-AP targets TSFM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSFM fusion protein Ag2308
• Full Name: Ts translation elongation factor, mitochondrial
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC022862
• Gene Symbol: TSFM
• Gene ID (NCBI): 10102
• RRID: AB_2207953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Biol Rep; Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells; Authors - Xiao-Yun Li ; KD Validated