Anticorps Polyclonal de lapin anti-MFF

• Phare
• Validé par KD/KO

MFF Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

168

Avis (2)

N° de cat : 17090-1-AP

Synonymes

C2orf33, MFF, mitochondrial fission factor

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Galerie de données de validation

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  WB analysis using 17090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17090-1-AP (MFF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17090-1-AP

  IP result of anti-MFF (IP:17090-1-AP, 4ug; Detection:17090-1-AP 1:30000) with mouse brain tissue lysate 1680 ug.

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17090-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFF antibody (17090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu d'estomac humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 153 publications below
• IHC: See 5 publications below
• IF: See 23 publications below
• IP: See 4 publications below

Informations sur le produit

17090-1-AP cible MFF dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, singe, souris, Hamster, squirrel
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MFF Protéine recombinante Ag9990
• Nom complet: mitochondrial fission factor
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC000797
• Symbole du gène: MFF
• Identification du gène (NCBI): 56947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Publications

Species	Application	Title
human	WB	Nature; Multiple dynamin family members collaborate to drive mitochondrial division.; Authors - Jason E Lee
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Nature; Correcting mitochondrial fusion by manipulating mitofusin conformations.; Authors - Antonietta Franco
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
monkey	WB	Science; ER tubules mark sites of mitochondrial division.; Authors - Jonathan R Friedman ; KD Validated
human	WB	Cell; CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity.; Authors - Philipp Hammerschmidt

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293 and HeLa

FH

JAE HO (Verified Customer) (04-22-2019)

I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: PC3/MDAMB231/LN229/MCF7/MCF10/DU145/LnCaP

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