DRP1 (N-terminal) Polyclonal antibody

DRP1 (N-terminal) Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 26187-1-AP

Synonyms

DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L

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Validation Data Gallery

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  WB analysis using 26187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26187-1-AP (DRP1 (N-terminal) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 26187-1-AP

  IP result of anti-DRP1 (N-terminal) (IP:26187-1-AP, 4ug; Detection:26187-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of HeLa using 26187-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DRP1 (N-terminal) antibody (26187-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

26187-1-AP targets DRP1 (N-terminal) in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRP1 (N-terminal) fusion protein Ag24249
• Full Name: dynamin 1-like
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC024590
• Gene Symbol: DRP1
• Gene ID (NCBI): 10059
• RRID: AB_2880417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	EMBO Mol Med; High-throughput screening identifies suppressors of mitochondrial fragmentation in OPA1 fibroblasts.; Authors - Emma Cretin
human	WB	Biochem Biophys Res Commun; DUSP1 overexpression attenuates renal tubular mitochondrial dysfunction by restoring Parkin-mediated mitophagy in diabetic nephropathy.; Authors - Chang Lu
human	WB	Asian J Androl; Mannose inhibits the growth of prostate cancer through a mitochondrial mechanism.; Authors - Yu-Lin Deng
	WB	Environ Pollut; Mitochondrial dynamics disruption: Unraveling Dinotefuran's impact on cardiotoxicity; Authors - Jiaping Zhu
mouse	WB	Inflamm Res; Transcriptomic analysis reveals molecular characterization and immune landscape of PANoptosis-related genes in atherosclerosis; Authors - Zhipeng Zheng