Anticorps Polyclonal de lapin anti-c-Met (Cytoplasmic)

• Phare
• Validé par KD/KO

c-Met (Cytoplasmic) Polyclonal Antibody for FC (intra), IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IP, IHC, IF, FC (intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications

43

N° de cat : 25869-1-AP

Synonymes

AUTS9, c-Met, c-Met (Cytoplasmic), HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

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Galerie de données de validation

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  WB analysis of HepG2 using 25869-1-AP

  WB result of c-Met antibody (25869-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met transfected HepG2 cells.

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  WB analysis using 25869-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25869-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDCK using 25869-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25869-1-AP

  IP Result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human lung cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 25869-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-Met (Cytoplasmic) (25869-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A431, cellules HepG2, cellules MDCK, tissu hépatique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 33 publications below
• IHC: See 10 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

25869-1-AP cible c-Met (Cytoplasmic) dans les applications de WB, IP, IHC, IF, FC (intra), CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: c-Met (Cytoplasmic) Protéine recombinante Ag23140
• Nom complet: met proto-oncogene (hepatocyte growth factor receptor)
• Masse moléculaire calculée: 1390 aa, 155 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: BC130420
• Symbole du gène: MET
• Identification du gène (NCBI): 4233
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Publications

Species	Application	Title
rat	WB	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
	WB	Oxid Med Cell Longev; PMID: 31781338; Authors - Ya-Dong Liu
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui
human	WB	Mol Ther Nucleic Acids; Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.; Authors - Yalei Cui
human	WB	Front Oncol; SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.; Authors - Ruoyan Liu

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