Anticorps Monoclonal anti-MBP tag
MBP tag Monoclonal Antibody for IF, IP, WB, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Protéine recombinante et plus (3)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
4C6H4
N° de cat : 66003-1-Ig
Synonymes
"MBP tag Antibodies" Comparison
View side-by-side comparison of MBP tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | Protéine recombinante |
| Résultats positifs en IP | protéine protéine recombinante |
| Résultats positifs en IF | cellules transfectées |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 25 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
Informations sur le produit
66003-1-Ig cible MBP tag dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | MBP tag Protéine recombinante Ag0942 |
| Nom complet | MBP tag |
| Masse moléculaire calculée | 40 kDa |
| Poids moléculaire observé | 40 kDa |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for MBP tag antibody 66003-1-Ig | Download protocol |
| IP protocol for MBP tag antibody 66003-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames | ||
Nat Commun Structural insights into pathogenic mechanism of hypohidrotic ectodermal dysplasia caused by ectodysplasin A variants | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Mol Cell Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination. | ||
Circ Res Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction. | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration |
