- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-MAP1B
MAP1B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugaison
Non conjugué
N° de cat : 21633-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu de cervelet de souris, tissu cérébral humain |
Résultats positifs en IHC | tissu cérébral de souris, tissu de cervelet de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF-P | tissu cérébral de souris, |
Résultats positifs en IF/ICC | cellules SH-SY5Y, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochimie (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 12 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Informations sur le produit
21633-1-AP cible MAP1B dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | MAP1B Protéine recombinante Ag16255 |
Nom complet | microtubule-associated protein 1B |
Masse moléculaire calculée | 2468 aa, 271 kDa |
Poids moléculaire observé | 320 kDa |
Numéro d’acquisition GenBank | BC141853 |
Symbole du gène | MAP1B |
Identification du gène (NCBI) | 4131 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)
Protocole
Product Specific Protocols | |
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WB protocol for MAP1B antibody 21633-1-AP | Download protocol |
IHC protocol for MAP1B antibody 21633-1-AP | Download protocol |
IF protocol for MAP1B antibody 21633-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Rep MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.
| ||
Cereb Cortex eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures. | ||
Front Cell Neurosci Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis | ||
Hum Mol Genet Microtubule associated protein 1B dysregulates microtubule dynamics and neuronal mitochondrial transport in Spinal Muscular Atrophy. | ||
Cell Cycle Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability. | ||
Neurochem Int FMRP-absence-induced up-regulation of hypothalamic MAP1B expression decreases AgRP level linking with reduces in food intake and body weight.
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Albane (Verified Customer) (02-18-2020) | A lot of bands, none at the correct size
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