• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-MAP1B

MAP1B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 21633-1-AP

Synonymes

Microtubule-associated protein 1B, MAP1B heavy chain, MAP-1B, MAP1 light chain LC1



Applications testées

Résultats positifs en WBtissu de cervelet de souris, tissu cérébral humain
Résultats positifs en IHCtissu cérébral de souris, tissu de cervelet de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu cérébral de souris,
Résultats positifs en IF/ICCcellules SH-SY5Y,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

21633-1-AP cible MAP1B dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MAP1B Protéine recombinante Ag16255
Nom complet microtubule-associated protein 1B
Masse moléculaire calculée 2468 aa, 271 kDa
Poids moléculaire observé 320 kDa
Numéro d’acquisition GenBankBC141853
Symbole du gène MAP1B
Identification du gène (NCBI) 4131
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

Protocole

Product Specific Protocols
WB protocol for MAP1B antibody 21633-1-APDownload protocol
IHC protocol for MAP1B antibody 21633-1-APDownload protocol
IF protocol for MAP1B antibody 21633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.

Authors - Kohei Arasaki
  • KD Validated
mouseWB

Cereb Cortex

eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.

Authors - Christopher Heise
mouseIF

Front Cell Neurosci

Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis

Authors - Yingzhen Zhang
mouseWB

Hum Mol Genet

Microtubule associated protein 1B dysregulates microtubule dynamics and neuronal mitochondrial transport in Spinal Muscular Atrophy.

Authors - Gamze Bora
humanWB

Cell Cycle

Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.

Authors - Junyu Wu
mouseWB,IF

Neurochem Int

FMRP-absence-induced up-regulation of hypothalamic MAP1B expression decreases AgRP level linking with reduces in food intake and body weight.

Authors - Jing-Yi Long
  • KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Albane (Verified Customer) (02-18-2020)

A lot of bands, none at the correct size

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: SH-SY5Y
MAP1B Antibody Western Blot, validation (1/1000 dilution) in SH-SY5Y (Cat no:21633-1-AP)