JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

159

Cat no : 24164-1-AP

Synonyms

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

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Validation Data Gallery

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  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24164-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24164-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24164-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 24164-1-AP

  IP result of anti-JNK (IP:24164-1-AP, 4ug; Detection:24164-1-AP 1:10000) with A431 cells lysate 1280 ug.

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  IHC staining of human colon cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 24164-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (24164-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 24164-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (24164-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 155 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

24164-1-AP targets JNK in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag21426
• Full Name: mitogen-activated protein kinase 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 42 kDa, 50 kDa
• GenBank Accession Number: BC130572
• Gene Symbol: JNK
• Gene ID (NCBI): 5599
• RRID: AB_2879443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Publications

Species	Application	Title
	WB	Mol Cell; PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis; Authors - Shouqiao Hou
human	WB	Adv Sci (Weinh); OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression; Authors - Xingran Li
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
mouse	WB	Transl Res; Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF.; Authors - Jiqian Zhao
human	WB	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
human	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang