MST1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MST1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 22245-1-AP

Synonyms

MST2, STK4, STK4/MST1, MST-1, MST 2

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Validation Data Gallery

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  WB analysis of HepG2 using 22245-1-AP

  WB result of MST1 antibody (22245-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

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  WB analysis using 22245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 22245-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22245-1-AP

  IP result of anti-MST1 (IP:22245-1-AP, 4ug; Detection:22245-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human prostate cancer using 22245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 22245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22245-1-AP

  Immunofluorescent analysis of HepG2 cells using 22245-1-AP (MST1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, rat liver tissue, Jurkat cells, Ramos cells, C2C12 cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

22245-1-AP targets MST1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MST1 fusion protein Ag17738
• Full Name: serine/threonine kinase 4
• Calculated Molecular Weight: 487 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC093768
• Gene Symbol: MST1
• Gene ID (NCBI): 6789
• RRID: AB_11182388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publications

Species	Application	Title
mouse	IF,WB	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
mouse	IHC	Cell Death Differ; Hippo kinases MST1 and MST2 control the differentiation of the epididymal initial segment via the MEK-ERK pathway.; Authors - Chenling Meng
mouse	WB,IHC	J Am Soc Nephrol; Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms.; Authors - Chunhua Xu ; KO Validated
human	WB,IHC,IF	Oncogene; Deacetylation of tumor-suppressor MST1 in Hippo pathway induces its degradation through HBXIP-elevated HDAC6 in promotion of breast cancer growth.; Authors - L Li
human	WB	Cell Death Differ; Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation.; Authors - Wenjiao Li