Anticorps Polyclonal de lapin anti-LGR5

LGR5 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 21833-1-AP

Synonymes

FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5

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Galerie de données de validation

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human colon cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21833-1-AP (LGR5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21833-1-AP (LGR5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:1000 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21833-1-AP cible LGR5 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGR5 Protéine recombinante Ag16389
• Nom complet: leucine-rich repeat-containing G protein-coupled receptor 5
• Masse moléculaire calculée: 907 aa, 100 kDa
• Numéro d’acquisition GenBank: BC096324
• Symbole du gène: LGR5
• Identification du gène (NCBI): 8549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LGR5, also named as GPR49, GPR67 and HG38, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. LGR5 is a stem cell marker of the intestinal epithelium and the hair follicule. It is a target gene of Wnt signaling.

Publications

Species	Application	Title
human	IHC	Oncotarget; LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma.; Authors - Gabriella Cunha Vieira
human	IF	Inflamm Bowel Dis; Overexpression of Cancer-Associated Stem Cell Gene OLFM4 in the Colonic Epithelium of Patients With Primary Sclerosing Cholangitis.; Authors - Mastura Neyazi
mouse	WB	J Dermatol Sci; REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells.; Authors - Xiao Gao
		Tumour Biol; Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas.; Authors - Yan Wang
		Oncol Lett; Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement.; Authors - Rei Ishibashi
human	IHC	Oncol Lett; Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer.; Authors - Ajay Kumar Gautam

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