LGR4 Polyclonal antibody

LGR4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20150-1-AP

Synonyms

G protein coupled receptor 48, GPR48, LGR4

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Validation Data Gallery

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  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 20150-1-AP is LGR4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGR4 fusion protein Ag13695
• Full Name: leucine-rich repeat-containing G protein-coupled receptor 4
• Calculated Molecular Weight: 104 kDa
• GenBank Accession Number: BC033039
• Gene Symbol: LGR4
• Gene ID (NCBI): 55366
• RRID: AB_2878648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LGR4, also known as GPR48, is a membrane of the leucine-rich GPCR family. LGR4 is composed of a large NH2-terminal extracellular domain containing 18 LRRs, the seven membrane-spanning segments, and a COOH-terminal intracellular domain. LGR4 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. It has also been shown that LGR4 regulates cyclic AMP/protein kinase A (PKA) signalling. (PMID: 23756652; 17178856; 24212090)

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB	Mol Ther Oncolytics; Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.; Authors - Xiaohong Ruan
human	IHC	Cancer Biomark; LGR4 overexpression is associated with clinical parameters and poor prognosis of serous ovarian cancer; Authors - Zhaoyang Zeng
human	IHC	Exp Cell Res; Silencing of RSPO1 mitigates obesity-related renal fibrosis in mice by deactivating Wnt/β-catenin pathway.; Authors - Xuesong Su
mouse	WB	In Vitro Cell Dev Biol Anim; SOX2 suppresses osteoblast differentiation of MC3T3-E1 cells through activating the transcription of LGR4; Authors - Sunyu Chen
pig,mouse	WB	Cell Death Dis; DNA methylation mediated RSPO2 to promote follicular development in mammals.; Authors - Xiaofeng Zhou