RSPO3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RSPO3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 17193-1-AP

Synonyms

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

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Validation Data Gallery

• 

  WB analysis using 17193-1-AP

  mouse small intestine were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 17193-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 17193-1-AP

  IP result of anti-RSPO3 (IP:17193-1-AP, 4ug; Detection:17193-1-AP 1:300) with human placenta tissue lysate 2800ug.

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, Transfected HEK-293 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

17193-1-AP targets RSPO3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSPO3 fusion protein Ag9899
• Full Name: R-spondin 3 homolog (Xenopus laevis)
• Calculated Molecular Weight: 272 aa, 31 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC022367
• Gene Symbol: RSPO3
• Gene ID (NCBI): 84870
• RRID: AB_2878360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Publications

Species	Application	Title
mouse	WB	Nat Commun; R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.; Authors - Teng Han
human	WB	Proc Natl Acad Sci U S A; RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.; Authors - Kendra S Carmon
mouse	WB,IF	Cell Death Dis; Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury; Authors - Ting-Tao Liu ; KD Validated
human	WB	Cardiovasc Res; Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis.; Authors - Bhupesh Singla
rat	WB	Heliyon; Astragalus polysaccharide improves diabetic ulcers by promoting M2-polarization of macrophages to reduce excessive inflammation via the β-catenin/ NF-κB axis at the late phase of wound-healing; Authors - Zhang Zhen
human	IHC	Am J Physiol Cell Physiol; R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.; Authors - Zhilong Chen

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