Anticorps Monoclonal anti-LC3A/B
LC3A/B Monoclonal Antibody for ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, souris et plus (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugaison
Non conjugué
CloneNo.
2B2B5
N° de cat : 66139-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Dilution recommandée
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 16 publications below |
| IHC | See 1 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Informations sur le produit
66139-1-Ig cible LC3A/B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris
| Réactivité | Humain, porc, souris |
| Réactivité citée | rat, Humain, porc, souris, Hamster |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Protéine recombinante |
| Nom complet | microtubule-associated protein 1 light chain 3 alpha |
| Masse moléculaire calculée | 14 kDa |
| Poids moléculaire observé | 14 kDa, 16 kDa |
| Numéro d’acquisition GenBank | NM_032514 |
| Symbole du gène | MAP1LC3A |
| Identification du gène (NCBI) | 84557 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
Publications
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Cell Death Dis Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy. | ||
Cell Prolif S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos. | ||
Mol Neurobiol Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy. | ||
Aging (Albany NY) Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs. | ||
Front Mol Neurosci Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models. |
