Beclin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Beclin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

917

Reviews (6)

Cat no : 11306-1-AP

Synonyms

BECN1, Beclin-1-C 37 kDa, Beclin-1-C 35 kDa, Beclin-1, Beclin1

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 11306-1-AP

  WB result of Beclin 1 antibody (11306-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beclin 1 transfected HEK-293 cells.

• 

  WB analysis using 11306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11306-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11306-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 11306-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11306-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11306-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDA-MB-453s using 11306-1-AP

  IP result of anti-Beclin 1 (IP:11306-1-AP, 4ug; Detection:11306-1-AP 1:1000) with MDA-MB-453s cells lysate 2800 ug.

• 

  IHC staining of human breast cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast hyperplasia using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 11306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 11306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 11306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 11306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 11306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 11306-1-AP

  Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse heart using 11306-1-AP

  Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of NIH/3T3 using 11306-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Beclin 1 antibody (11306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, HEK-293 cells, Raji cells, L02 cells, PC-3 cells, A431 cells, HT-1080 cells, human placenta tissue, mouse brain tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, MDA-MB-453s cells, SH-SY5Y cells
• Positive IP detected in: MDA-MB-453s cells
• Positive IHC detected in: human breast cancer tissue, human breast hyperplasia tissue, human prostate hyperplasia tissue, human cervical cancer tissue, human colon cancer tissue, human stomach tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF-Fro detected in: mouse heart tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 21 publications below
• WB: See 836 publications below
• IHC: See 103 publications below
• IF: See 74 publications below
• IP: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 8 publications below

Product Information

11306-1-AP targets Beclin 1 in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, hamster, goat, fish, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beclin 1 fusion protein Ag1843
• Full Name: beclin 1, autophagy related
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: BC010276
• Gene Symbol: Beclin 1
• Gene ID (NCBI): 8678
• RRID: AB_2259061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

Publications

Species	Application	Title
human	WB	Cell; TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival; Authors - Pauline Lascaux
human	WB,IP	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
mouse	IF	ACS Nano; Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia; Authors - Shishi Shen
human	WB,IHC	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB,IF	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-30-2022)

This antibody works well in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

Beclin 1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:11306-1-AP)

FH

Jonathan (Verified Customer) (04-08-2022)

Antibody worked well - gave a nice, specific and strong signal. Happy.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: Vero cells (African Green Monkey)

FH

Zhengnan (Verified Customer) (02-24-2022)

The antibody is very good, got correct bands and background is clear!

• Applications: Western Blot

FH

David (Verified Customer) (03-30-2020)

Used for immunoblot, single band at expected molecular weight, good signal to noise ratio

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: SH-SY5Y

FH

Kanika (Verified Customer) (09-18-2019)

Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human platelets

Beclin 1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in Human platelets (Cat no:11306-1-AP)

FH

Kishor (Verified Customer) (01-30-2019)

Working well for Western Blotting (1:5000) and IF (1:300)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

Beclin 1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:11306-1-AP)

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