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- KD/KO Validated
GABARAPL2-Specific Polyclonal antibody
GABARAPL2-Specific Polyclonal Antibody for FC, IHC, WB,ELISA
Cat no : 18724-1-AP
Validation Data Gallery
|Positive WB detected in
|COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
|Positive IHC detected in
|human heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:20-1:200
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18724-1-AP targets GABARAPL2-Specific in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GABA(A) receptor-associated protein-like 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.
|Product Specific Protocols
|WB protocol for GABARAPL2-Specific antibody 18724-1-AP
|IHC protocol for GABARAPL2-Specific antibody 18724-1-AP
|FC protocol for GABARAPL2-Specific antibody 18724-1-AP
|Click here to view our Standard Protocols
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.
CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.
Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.
Comput Biol Med
Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data
Cell Death Dis
Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.
Alteration of autophagosomal proteins in the brain of multiple system atrophy.