• Featured Product
  • KD/KO Validated

GABARAPL2-Specific Polyclonal antibody

GABARAPL2-Specific Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 18724-1-AP

Synonyms

ATG8, FLC3A, GABARAPL2, GATE 16, GATE16, GEF 2, GEF2



Tested Applications

Positive WB detected inCOLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18724-1-AP targets GABARAPL2-Specific in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GABA(A) receptor-associated protein-like 2
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005985
Gene symbol GABARAPL2
Gene ID (NCBI) 11345
RRIDAB_2232116
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Protocols

Product Specific Protocols
WB protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
IHC protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
FC protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Immunol

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Authors - Miwa Sasai
  • KO Validated
ratWB

Autophagy

CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.

Authors - Hyun-Kyu An
humanWB

Autophagy

Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.

Authors - Jonathan A Stefely
humanIHC

Comput Biol Med

Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data

Authors - Zehua Wang
humanWB

Cell Death Dis

Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.

Authors - Christos E Zois
humanWB, IHC

Neurobiol Dis

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Authors - Tanji Kunikazu K