GABARAPL2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

GABARAPL2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 18724-1-AP

Synonyms

ATG8, FLC3A, GABARAPL2, GABARAPL2-Specific, GATE 16, GATE16, GEF 2, GEF2

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 18724-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18724-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18724-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 18724-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18724-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL2-Specific antibody (18724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
• Positive IHC detected in: human heart tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Chloroquine treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

18724-1-AP targets GABARAPL2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GABA(A) receptor-associated protein-like 2
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC005985
• Gene Symbol: GABARAPL2
• Gene ID (NCBI): 11345
• RRID: AB_2232116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai ; KO Validated
rat	WB	Autophagy; CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.; Authors - Hyun-Kyu An
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
human	IHC	Comput Biol Med; Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data; Authors - Zehua Wang
human	WB	Cell Death Dis; Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.; Authors - Christos E Zois
human	WB, IHC	Neurobiol Dis; Alteration of autophagosomal proteins in the brain of multiple system atrophy.; Authors - Tanji Kunikazu K

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