Anticorps Polyclonal de lapin anti-LAMP3

• Phare
• Validé par KD/KO

LAMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 12632-1-AP

Synonymes

CD208, LAMP 3, LAMP, DC-lysosome-associated membrane glycoprotein, DCLAMP

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Galerie de données de validation

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  WB analysis using 12632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 12632-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12632-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12632-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12632-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12632-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12632-1-AP

  IP result of anti-LAMP3 (IP:12632-1-AP, 4ug; Detection:12632-1-AP 1:500) with A549 cells lysate 2800ug.

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  IHC staining of human lung using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 12632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAMP3 antibody (12632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A549, cellules HEK-293T, cellules Raji, tissu de thymus de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu pulmonaire humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 10 publications below

Informations sur le produit

12632-1-AP cible LAMP3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAMP3 Protéine recombinante Ag3325
• Nom complet: lysosomal-associated membrane protein 3
• Masse moléculaire calculée: 416 aa, 44 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC032940
• Symbole du gène: LAMP3
• Identification du gène (NCBI): 27074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).

Publications

Species	Application	Title
human	WB	Nat Med; Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.; Authors - Yingxiao Shi
human	IF	Arthritis Rheumatol; LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands; Authors - Hiroyuki Nakamura
monkey	WB	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
human	WB,IF	J Clin Invest; Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.; Authors - Ying-Qian Mo
human	WB,IF	Autophagy; LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.; Authors - Tsutomu Tanaka
mouse	IF	Cell Prolif; Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.; Authors - Xiaofan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ying (Verified Customer) (08-19-2019)

very good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse lung tissues

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