• Featured Product
  • KD/KO Validated

CD107b / LAMP2 Monoclonal antibody

CD107b / LAMP2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgA


human, mouse, pig and More (2)







Cat no : 66301-1-Ig


CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

Tested Applications

Positive WB detected inL02 cells, pig liver tissue, mouse liver tissue, HEK-293 cells, HUVEC cells, Jurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue, human lung cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, 3T3-L1 cells, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66301-1-Ig targets CD107b / LAMP2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, chicken, pig, bovine
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen CD107b / LAMP2 fusion protein Ag0595
Full Name lysosomal-associated membrane protein 2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.


Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 66301-1-IgDownload protocol
IHC protocol for CD107b / LAMP2 antibody 66301-1-IgDownload protocol
IF protocol for CD107b / LAMP2 antibody 66301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Authors - Xinjian Liu

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang


SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng

Arthritis Rheumatol

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Authors - Hiroyuki Nakamura

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang

Redox Biol

WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury

Authors - Zebin Deng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Laura (Verified Customer) (04-08-2024)

Antibody works nicely in ICC-IF and WB in different cell lines of intestine and pancreas origin.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: As specified in product datasheet

LENIN (Verified Customer) (06-12-2022)

Antibody works very well for culture cells and Western blots.

  • Applications: Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: LIVER CELLS
CD107b / LAMP2 Antibody Cell culture validation (1:1000 dilution) in LIVER CELLS (Cat no:66301-1-Ig)

NYDIA (Verified Customer) (03-12-2022)

This antibody works great for immunofluorescence. No background. I highly recommend this product.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa cells

Eric (Verified Customer) (03-13-2021)

I had success with some and others not. I used microglia and I did not get strong expression but in macrophages I got better bands with same dilution and treatment. Many nonspecific bands

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Microglia/Macrophages
CD107b / LAMP2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia/Macrophages (Cat no:66301-1-Ig)

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain

miguel (Verified Customer) (12-11-2018)

The antibody was used with an inhouse protocol for Immunohistochemistry. The results varied from good to great for both stains and imaging.

  • Applications: Western Blot, Immunohistochemistry,
  • Cell Tissue Type: Rat Brain