Anticorps Polyclonal de lapin anti-Cytokeratin 14

• Phare
• Validé par KD/KO

Cytokeratin 14 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

68

Avis (3)

N° de cat : 10143-1-AP

Synonymes

Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Galerie de données de validation

• 

  WB analysis of A431 using 10143-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 10143-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10143-1-AP

  IP result of anti-Cytokeratin 14 (IP:10143-1-AP, 3ug; Detection:10143-1-AP 1:1000) with A431 cells lysate 1200ug.

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human breast cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:400 (under 40x lens).

• 

  IF Staining of HepG2 using 10143-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HaCaT using 10143-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10143-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

  IF result of CK14 (10143-1-AP, 1:400) with 1% PLP fixed basal quiescent stem cells of the adult mouse olfactory epithelium. (Green: CK14; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.

• 

  FC experiment of A431 using 10143-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 14 (10143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, tissu cutané de rat
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cutané de souris, tissu cutané humain, tissu de cancer de la peau humain, tissu de cancer de l'œsophage humain, tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HaCaT, cellules HeLa, cellules HepG2, tissu d'épithélium olfactif de souris
• Résultats positifs en FC (Intra): cellules A431
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 22 publications below
• IF: See 41 publications below

Informations sur le produit

10143-1-AP cible Cytokeratin 14 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 14 Protéine recombinante Ag0188
• Nom complet: keratin 14
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC002690
• Symbole du gène: Cytokeratin 14
• Identification du gène (NCBI): 3861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Publications

Species	Application	Title
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Nat Commun; Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.; Authors - Yue Hong
human	IF	Nat Commun; Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.; Authors - Vlasta Lungova
mouse	IF	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
human	WB	Oncogene; EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.; Authors - C-F Yang
mouse	IF	Aging Cell; Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.; Authors - Wen-Li Hsu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rachel (Verified Customer) (03-19-2024)

Excellent antibody, got the tester but will be purchasing more for future work.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: MCF10A

Cytokeratin 14 Antibody Western Blot,Immunofluorescence validation (1:200 dilution) in MCF10A (Cat no:10143-1-AP)

FH

Parmveer (Verified Customer) (10-20-2019)

Worked well to detect basal cells around the prostate gland of formalin fixed tissue

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Prostate

Cytokeratin 14 Antibody Immunofluorescence, validation (1:400 dilution) in Prostate (Cat no:10143-1-AP)

FH

Joshua (Verified Customer) (05-10-2019)

Human Keratinocytes fixed in 4% paraformaldehyde and stained for KRT14. Goat anti-rabbit 488. Bright, specific staining observed.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Cytokeratin 14 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:10143-1-AP)

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