Cytokeratin 15 Polyclonal antibody

Cytokeratin 15 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 10137-1-AP


CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

Tested Applications

Positive WB detected inA431 cells, mouse thymus tissue, HeLa cells, PC-3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10137-1-AP targets Cytokeratin 15 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185
Full Name keratin 15
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002641
Gene Symbol KRT15
Gene ID (NCBI) 3866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.


Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IHC protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IP protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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