Anticorps Polyclonal de lapin anti-CD117/c-Kit

CD117/c-Kit Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 18696-1-AP

Synonymes

C Kit, CD117, c-Kit, c-Kit/CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Galerie de données de validation

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  WB analysis of Mo7e using 18696-1-AP

  Mo7e cells were subjected to SDS PAGE followed by western blot with 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (CD117/c-kit antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (CD117/c-kit antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human testis using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of MCF-7 using 18696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: Mo7e cells,
• Résultats positifs en IHC: tissu stroma tumoral, tissu d'insulinome, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 8 publications below

Informations sur le produit

18696-1-AP cible CD117/c-Kit dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: CD117
• Identification du gène (NCBI): 3815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Publications

Species	Application	Title
human	WB	Mech Ageing Dev; Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy; Authors - Bing-Qi Dong
human	IHC	Front Cell Dev Biol; Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.; Authors - Xinwei Lin
human	IHC	Front Immunol; Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment.; Authors - Nan Zhang
human	IF	J Exp Clin Cancer Res; Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.; Authors - Wei Lu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen
goat	IF	J Anim Sci; A novel regulatory sex-skewing method that inhibits testicular DPY30 expression to increase female rate of dairy goat offspring; Authors - Huanshan He

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