Anticorps Polyclonal de lapin anti-Rubicon

• Phare
• Validé par KD/KO

Rubicon Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 21444-1-AP

Synonymes

Baron, KIAA0226, RUBCN, Rubicon

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Galerie de données de validation

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  WB analysis of mouse liver using 21444-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21444-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 21444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Rubicon antibody (21444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 21444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Rubicon antibody (21444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu testiculaire humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below

Informations sur le produit

21444-1-AP cible Rubicon dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Rubicon Protéine recombinante Ag13715
• Nom complet: KIAA0226
• Masse moléculaire calculée: 972 aa, 109 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC033615
• Symbole du gène: Rubicon
• Identification du gène (NCBI): 9711
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rubicon (Run domain Beclin-1 interacting and cysteine-rich containing protein) consists of an N-terminal RUN domain, a middle region that includes its PI3K-binding domain (PIKBD), a coiled-coil domain (CCD), a serine-rich region (S-R), a helix-coil-rich domain (H-C) and a C-terminal Rubicon homology domain (RH) (PMID: 36288306). Rubicon is ubiquitously expressed in most tissue and organs.

Publications

Species	Application	Title
mouse	WB,IF	ACS Nano; Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia; Authors - Shishi Shen
human	WB,IF,IP	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou
		Genes Dev; USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.; Authors - Daichao Xu
rat	WB,IF	Autophagy; RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.; Authors - Luis Muniz-Feliciano ; KD Validated
human	WB,IHC	Cell Mol Immunol; PMID: 28392573; Authors - Yushun Wan ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (07-21-2021)

The antibody seemed to work okay, although I had issues differentiating between autofluorescence and a positive signal. However, that is not the fault of the antibody, but rather my cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: iBMDMs

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