Anticorps Polyclonal de lapin anti-IGFBP3

• Phare
• Validé par KD/KO

IGFBP3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

30

Avis (1)

N° de cat : 10189-2-AP

Synonymes

BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

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Galerie de données de validation

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  WB analysis of mouse colon using 10189-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10189-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10189-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10189-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10189-2-AP

  Immunofluorescent analysis of HeLa cells using 10189-2-AP (IGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, cellules A2780, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 6 publications below
• IF: See 7 publications below

Informations sur le produit

10189-2-AP cible IGFBP3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGFBP3 Protéine recombinante Ag0242
• Nom complet: insulin-like growth factor binding protein 3
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-37 kDa
• Numéro d’acquisition GenBank: BC000013
• Symbole du gène: IGFBP3
• Identification du gène (NCBI): 3486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

Publications

Species	Application	Title
human	IF	Cell; 3D reconstruction of a gastrulating human embryo; Authors - Zhenyu Xiao
human,mouse	WB,IHC,IF	Theranostics; Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p; Authors - Soo Hyun Kang ; KD Validated
		Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
mouse	WB	Acta Physiol (Oxf); Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.; Authors - Yingying Zhang
human	WB	Cancer Lett; Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.; Authors - Yuan Yin
mouse	WB	PLoS Biol; The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.; Authors - Patrick A Carroll

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Margarita (Verified Customer) (04-19-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: PC3 cells

IGFBP3 Antibody Immunofluorescence validation (1:100 dilution) in PC3 cells (Cat no:10189-2-AP)

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