Tested Applications
| Positive WB detected in | HeLa cells, L02 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20254-1-AP targets IGF1R/CD221 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | IGF I receptor |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | 100, 130 kDa |
| GenBank Accession Number | NM_000875 |
| Gene Symbol | IGF1R |
| Gene ID (NCBI) | 3480 |
| RRID | AB_10667455 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08069 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGF1R/CD221 antibody 20254-1-AP | Download protocol |
| IP protocol for IGF1R/CD221 antibody 20254-1-AP | Download protocol |
| WB protocol for IGF1R/CD221 antibody 20254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-IGF1R (Insulin-like Growth Factor 1 Receptor), catalog number 20254-1-AP, has 71 total citations. Publications appear in journals ranging from top-tier venues such as Cell, Cancer Cell, Nature Biotechnology, and Molecular Cell to specialized journals including Gastroenterology, Oncogene, FASEB Journal, and Frontiers in Immunology. Associated research fields encompass oncology (lung, pancreatic, ovarian, breast, colorectal, prostate cancers), neurodegeneration, metabolic disorders (diabetes, obesity), immunology, cardiovascular disease, reproductive biology, aging, and toxicology.
| Species | Application | Title |
|---|---|---|
Cancer Cell Mapping cell type-resolved transcriptomic profiles to patient survival in pancreatic cancer. | ||
Cell QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism | ||
Mol Cancer CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis. | ||
Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1. | ||
Sci Bull (Beijing) Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes |
Reviews
What customers say
One reviewer (Tongbin Wu) rated this antibody 3 out of 5 stars in a Western Blot application on heart tissue. They reported a strong and specific IGF1R band but noted the presence of some faint non-specific bands. Overall, the antibody performed well for its target but showed minor background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-25-2020) | This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.
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