IGF1R/CD221 Polyclonal antibody

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  WB analysis of HeLa using 20254-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20254-1-AP (IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20254-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20254-1-AP (IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20254-1-AP

  IP result of anti-IGF1R (IP:20254-1-AP, 4ug; Detection:20254-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IHC staining of human lung cancer using 20254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20254-1-AP (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 20254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20254-1-AP (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

IGF1R/CD221 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20254-1-AP

Publications(57)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 49 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

20254-1-AP targets IGF1R/CD221 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: IGF I receptor
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 100, 130 kDa
• GenBank Accession Number: NM_000875
• Gene Symbol: IGF1R
• Gene ID (NCBI): 3480
• RRID: AB_10667455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08069
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Sci Bull (Beijing); Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes; Authors - Jiangbing Xiang
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB	Mol Cancer; CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis.; Authors - Jinghan Hua
mouse	WB	J Cachexia Sarcopenia Muscle; Exogenous insulin-like growth factor 1 attenuates cisplatin-induced muscle atrophy in mice.; Authors - Hiroyasu Sakai
mouse	WB,IF	Cell Death Dis; Insulin-like growth factor 1 receptor mediates photoreceptor neuroprotection; Authors - Ammaji Rajala
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.

• Applications: Western Blot
• Cell Tissue Type: Heart