Anticorps Polyclonal de lapin anti-IGF2BP3

• Phare
• Validé par KD/KO

IGF2BP3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

103

Avis (2)

N° de cat : 14642-1-AP

Synonymes

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

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Galerie de données de validation

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  WB analysis using 14642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 14642-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14642-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14642-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14642-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14642-1-AP

  IP result of anti-IGF2BP3 (IP:14642-1-AP, 5ug; Detection:14642-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.

• 

  IHC staining of human pancreas using 14642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14642-1-AP

  Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules BxPC-3, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu cérébral de souris, tissu placentaire de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu pancréatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 37 publications below
• WB: See 82 publications below
• IHC: See 27 publications below
• IF: See 14 publications below
• IP: See 8 publications below
• CoIP: See 1 publications below
• RIP: See 20 publications below

Informations sur le produit

14642-1-AP cible IGF2BP3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGF2BP3 Protéine recombinante Ag6226
• Nom complet: insulin-like growth factor 2 mRNA binding protein 3
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC065269
• Symbole du gène: IGF2BP3
• Identification du gène (NCBI): 10643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Publications

Species	Application	Title
human	WB	Cell; SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.; Authors - Sita Kugel
mouse	WB	Cancer Cell; Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.; Authors - Liem H Nguyen ; KD Validated
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	RIP	Nat Cell Biol; N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.; Authors - You Wu
human	WB	Mol Cell; m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3; Authors - Ting Shan ; KD Validated
human	WB,RIP	Mol Cancer; Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification.; Authors - Yiyang Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

honglei (Verified Customer) (01-17-2023)

This antibody can be used for immunofluorescence and mainly localizes in the nucleus

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: cell line

FH

Jessica (Verified Customer) (10-29-2018)

Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: Neuroblastoma cells (human)

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