RAB27A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB27A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

59

Cat no : 17817-1-AP

Synonyms

Rab-27, RAB27, Rab 27, GTP-binding protein Ram, GTP binding protein Ram

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Validation Data Gallery

• 

  WB analysis using 17817-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K562 using 17817-1-AP

  WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells.

• 

  WB analysis of L02 using 17817-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17817-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17817-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17817-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17817-1-AP

  IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug.

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  IHC staining of human malignant melanoma using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, human liver tissue, HeLa cells, L02 cells, K-562 cells, K562, Jurkat cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human malignant melanoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 54 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Product Information

17817-1-AP targets RAB27A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, pig, canine, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB27A fusion protein Ag12136
• Full Name: RAB27A, member RAS oncogene family
• Calculated Molecular Weight: 221 aa, 25 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC107680
• Gene Symbol: RAB27A
• Gene ID (NCBI): 5873
• RRID: AB_2176728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Adv Sci (Weinh); Exosome Release Is Regulated by mTORC1.; Authors - Wenchong Zou
human	WB	J Allergy Clin Immunol; A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.; Authors - Bianca Tesi
human	WB	Genes Dev; Cdx2 regulates endo-lysosomal function and epithelial cell polarity.; Authors - Gao Nan N
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi ; KD Validated
		Blood; Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells.; Authors - Dawn T Smallwood

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