Anticorps Polyclonal de lapin anti-GRP94

• Phare
• Validé par KD/KO

GRP94 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

41

N° de cat : 14700-1-AP

Synonymes

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

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Galerie de données de validation

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  WB analysis using 14700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14700-1-AP (GRP94 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14700-1-AP

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 14700-1-AP (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14700-1-AP

  IP result of anti-GRP94 (IP:14700-1-AP, 3ug; Detection:14700-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IP experiment of mouse liver using 14700-1-AP

  IP result of anti-GRP94 (IP:14700-1-AP, 4ug; Detection:14700-1-AP 1:2000) with mouse liver tissue lysate 1560 ug.

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  IHC staining of human breast cancer using 14700-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 14700-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 14700-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 14700-1-AP (HSP90B1 Antibody) at dilution of 1:50.

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  IHC staining of human cervical cancer using 14700-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 14700-1-AP (HSP90B1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 14700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP94 antibody (14700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A431 using 14700-1-AP

  Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (14700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 14700-1-AP

  Immunofluorescent analysis of HepG2 cells, using HSP90B1 antibody 14700-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 14700-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (14700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules U2OS
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 39 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14700-1-AP cible GRP94 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRP94 Protéine recombinante Ag6344
• Nom complet: heat shock protein 90kDa beta (Grp94), member 1
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC066656
• Symbole du gène: GRP94
• Identification du gène (NCBI): 7184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Publications

Species	Application	Title
human	WB	Cell; A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.; Authors - Min Zhang
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
human	WB,IF	Theranostics; Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.; Authors - Zhiwei Liao
rat	WB	Redox Biol; Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.; Authors - Yuehong Wang
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik
mouse	WB	Antioxid Redox Signal; PMID: 27565538; Authors - Tianhe Li

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